Determination of triterpenoid saponin and polysaccharide content from in vitro cultures of Astragalus mongholicus Bunge

In this study, the efficient micropropagation protocol of Astragalus mongholicus Bunge was established and also triterpenoid saponin polysaccharide content in ethanol, methanol aqueous extracts different samples were determined by using spectrophotometric methods to investigate whether biologically active compounds depends on stage development plant during vitro culture. The total saponins polysaccharides cultures A. grown higher (990 505 μg/ml) ethanol extracted 14-day-old young shoot than 28-day-old propagated rooted shoots.